Innovation and research for fluorescence microscopy
Spirochrome
Innovative fluorescent probes
Imaging of biological structures in living cells is increasingly popular and needed. Opposite to immunostaining of fixed cells, live cell fluorescence microscopy requires unique fluorescent reporters. Those need to be able to label living cells either by crossing the cellular membranes or by being assembled within the cell (like GFP).
Spirochrome’s mission is to provide the scientific community with innovative and disruptive probes in the field of live cell fluorescence imaging. They’re committed and proud to offer reliable and high quality live cell imaging fluorescent probes carefully designed and optimized to work with the utmost simple staining protocol: “add and image”. They continuously put a lot of effort in the development of new fluorophores and innovative tools that will advance your research.
Spirochrome’s technology is based on silicon rhodamine (SiR) and SPY™ fluorophores1,3). These are bright and photostable rhodamine-like dye. Their key features are cell permeability, high photostability, fluorogenic character and compatibility with super-resolution microscopy. The fluorescence excitation and emission of SiR and SPY™ fluorophores cover the whole visible spectrum. SiR and SPY™ fluorophores are compatible with most microscopes usually falling within usual settings such as Cy3, Cy5 or FITC. They use their SiR and SPY™ fluorophores in conjunction with ligands binding to cellular structures such as DNA, actin, microtubules or lysosomes to create reliable and high quality probes for live cell imaging. This combination set SiR and SPY™ based probes at the leading edge of high performance & high quality fluorescent probes.
Membrane Tension Probes
Fluorescent dyes for precise measurement of cell membrane tension, enabling real-time monitoring and high-resolution visualisation of cellular mechanics.
SiR Probes
State-of-the-art fluorescent dyes enabling high-resolution live-cell imaging, providing exceptional versatility and sensitivity for a wide range of biological applications.
SPY Probes
Uniquely sensitive and versatile fluorescent dyes for precise cellular measurement and visualisation, enabling in-depth exploration of complex cellular dynamics with exceptional accuracy and efficiency
SiR700 Probes
Cutting-edge fluorescent dyes optimized for near-infrared imaging, providing exceptional sensitivity and resolution for live-cell and deep tissue applications, revolutionizing the field of biological imaging.
PKMito Probes
Cutting-edge fluorescent dyes for targeted mitochondrial imaging, offering precise visualization and analysis of mitochondrial function and dynamics.
SiR Fluorophores and Reagents
State-of-the-art fluorescent probes and reagents that utilise the silicon-rhodamine (SiR) chemistry for precise and sensitive imaging of live cells.